Hybrid programs blend both competency- and time-based strategies,
using a minimum and maximum range of hours for each major job
function and the successful demonstration of identified competencies.
using a minimum and maximum range of hours for each major job
function and the successful demonstration of identified competencies.
Medical Laboratory Technician
Anonymous
North Carolina (SAA)
Documents
View Redacted Document
Personal and employer info redacted
Individual state requirements may vary. Please contact your local apprenticeship office to ensure this version is suitable to your state’s requirements.
Work Process Content
On the Job Training
Anonymous
272
Skills
Blood Bank
49
Blood Bank
49
- Receive samples, check files
- Store, retain samples (discuss)
- Dispense blood, components, RhlG
- Indications for blood component transfusion (discuss)
- Return blood and components
- Emergency issue blood discuss
- Platelets
- Thaw FFP
- Thaw/P001 cryoprecipitate (discuss)
- Wash red blood cells
- Deglycerolize red blood cells (discuss)
- Split and pack red cells (discuss)
- Irradiate components (discuss)
- Reagent quality control
- Alarm checks, temperatures, inspection (discuss)
- Equipment maintenance (discuss)
- Receipt/processing of blood/components
- ABO/Rh blood typing, Al lectin typing
- Du testing (Weak D Testing)
- Rh phenotyping
- Direct Antiglobulin Tests (DAT)
- Workup positive DAT/monospecific reagents
- Elution and anel on eluate discuss
- Antibody detection tests (Screen)
- Positive antibody detection tests
- Antibody identification panels
- Enhancements (Peg, enzyme) (discuss)
- Compatibility test
- Type and screen
- Incompatible crossmatch
- Antigen typing
- Transfusion reaction workup (discuss)
- Blood/component inventory (discuss)
- Special tested components/CMV-, HgbS- (discuss)
- SPecial filters/Leukocyte reduction discuss
- Prenatal testing (discuss)
- Fetal bleed screen (discuss)
- Kleihauer-Betke test (discuss)
- Calculation of RHIG dosa e discuss
- Postnatal testing
- Cord blood testing
- Donor selection/interview/physical (discuss)
- Donor blood collection (discuss)
- Donor reactions/treatment (discuss)
- Donor blood procession/testing (discuss)
- Donor blood labeling (discuss)
- Directed donations (discuss)
- Autologous donations (discuss)
- Therapeutic phlebotomy (discuss)
Clinical Chemistry
65
Clinical Chemistry
65
- Reviews QC and preventive maintenance procedure for centrifuges.
- Reviews QC and preventive maintenance procedure for refrigerators
- States corrective action to take when refrigerators temperatures exceed determined limits.
- Reviews QC and preventive maintenance procedure for each chemistry analyzer
- Evaluates reagents, calibrators, standards and controls for suitability: out-dating, appearance
- Select correct pipette for reagent and sample preparation.
- Prepares reagents, calibrators, standards and controls in accordance with manufacturer' s directions
- Analyzes control reagent according to manufacturer' s instructions
- Verifies that quality control results are within +2 standard deviations of pre- determined limits.
- Takes corrective action for controls that do not meet predetermined limits.
- Performs correlation studies to bring new lot numbers of control and/or reagents into service. (If applicable)
- Performs linearity studies of equipment. (Discuss)
- Uses Westguard rules to evaluate control values
- Evaluates Levy-Jennings charts for shifts and trends.
- Prioritizes samples based on urgency of test requests.
- Correctly identifies patient sample using two identifiers.
- States the reason for rejection of samples according to department protocol
- State the sample types acceptable, i.e., EDTA, Clot, for each test performed in the clinical chemistry department.
- Evaluates samples for suitability for use: additive, QNS, etc.
- Identifies pre-analytical errors which will interfere with specific tests, i.e., hemolysis, lipemia, icterus, incorrect tube additive,
- Separates serum or plasma in an appropriate manner.
- Properly prepares specimens for shipment to reference laboratories.
- Labels transfer tubes completely and accurately.
- Stores samples at appropriate conditions.
- Accurately performs dilutions using appropriate ratios and proper diluting fluid.
- Accurately performs creatinine clearance calculation.
- Analyzer One: States principle of the instrument operation or test procedure reaction
- Analyzer One: Startup procedure – calibrations (when required)
- Analyzer One: Daily maintenance required.
- Analyzer One: Other required scheduled maintenance.
- Analyzer One: Record keeping for operational and QC procedures
- Analyzer One: Routine instrument operation.
- Analyzer One: Troubleshooting techniques
- Analyzer Two: States principle of the instrument operation or test procedure reaction
- Analyzer Two:Startup procedure – calibrations (when required)
- Analyzer Two: Daily maintenance required.
- Analyzer Two: Other required scheduled maintenance.
- Analyzer Two: Record keeping for operational and QC procedures
- Analyzer Two: Routine instrument operation.
- Analyzer Two: Troubleshooting techniques
- Make/model of analyzer used:
- Make/model of analyzer used:
- Make/model of analyzer used:
- Make/model of analyzer used:
- Make/model of analyzer used:
- Make/model of analyzer used:
- Make/model of analyzer used:
- Selects and prepares appropriate sample for testing.
- Determines concentration of unknown samples.
- Report results using appropriate units.
- Identify results out of instrument sensitivity range.
- Appropriately acts on out of range results by reporting to preceptor
- Interpret results as to normal or abnormal based on normal values utilized by clinical site.
- Correlates clinical significance of abnormal results obtained to possible disease conditions.
- States additional tests to perform when abnormal results are obtained.
- Recognizes critical values
- Appropriately acts on critical values by reporting to preceptor.
- Nephelometry *
- Turbidometry*
- Chromatography*
- Electrophoresis*
- Immunodiffusion*
- Osmometry*
- Chemluminescence
- Other (Identify)
Coagulation
21
Coagulation
21
- Locate and review coagulation manual
- Process incoming specimens for testing
- Run controls on the coagulation analyzer and determine proper course of action if out of range
- Run patients on the coagulation analyzer
- Run patients and controls on backup coagulation analyzer
- Perform departmental QC and record (temperatures, etc.)
- Maintain proper departmental paperwork
- Document appropriate course of action if controls are out of range
- Interpret routine coagulation studies
- Troubleshoot coagulation procedural problems
- Mixing Studies
- Platelet Function Assays
- Heparin Testing
- Discuss the principles, operations and routine maintenance of primary and backup coagulation analyzers
- Discuss the factors involved in a PT and PTT test
- Discuss the reagents and specimens used in coagulation testing, specifically PT and PTT
- Discuss interference with results of PT and PTT
- Discuss procedures for monitoring anticoagulant therapy using PT (INR) and PTT testing
- Discuss additional coagulation testing such as factor assays
- Discuss principles of fibrinogen assay
- Discuss D-dimer
Hematology
64
Hematology
64
- Refrigerator(s), i.e., temperatures.
- Reviews QC and preventative maintenance procedure for refrigerators.
- States corrective action to take when refrigerator’s temperature exceed predetermined limits.
- Reviews QC and preventative maintenance procedure for each hematology analyzer.
- Verifies that quality control results are with set limits.
- Performs and analyzes QC according to manufacturer’s instructions.
- Evaluates reagents, calibrators, standards, and controls for suitability, including outdating and appearance.
- Prepares reagents, calibrators, standards, and controls in accordance with manufacturer’s directions.
- Labels controls appropriately.
- Properly records and/or stores QC values according to protocol.
- Verifies that quality control results are within ± 2 standard deviations of pre-determined limits.
- Takes corrective action for controls that do not meet predetermined limits, using Westguard rules to interpret control values.
- Monitors Levy-Jennings charts for shifts and trends.
- Discuss comparison/correlation studies to bring new lot numbers of control and/or reagents into service.
- Discuss linearity studies of equipment
- Other :
- Prioritizes samples based on urgency of test requests.
- Correctly identifies patient sample using 2 identifiers.
- State the sample types acceptable for each test performed in the hematology department.
- States the reason for rejection of samples according to department protocol.
- Evaluates samples for suitability for use: clots, additive, QNS, etc.
- Identifies pre-analytical errors which will interfere with specific tests, i.e., high hematocrits, hemolysis, lipemia, incorrect tube additive, etc.
- Stores samples at appropriate conditions.
- Preparation of 10 blood smears that exhibit acceptable degrees of cell distribution.
- Stain 10 blood smears with Wright’s stain so that the cells are characteristically stained and identifiable.
- Read/discuss principle of the instrument operation or test procedure reaction.
- Perform instrument startup procedure.
- Perform instrument shutdown procedure.
- Performs daily maintenance.
- Performs other required scheduled maintenance.
- Record keeping for operational and QC procedures.
- Operates instrumentation properly and accurately.
- Discuss common troubleshooting techniques.
- Evaluates 25 histograms/scatterplots for accuracy.
- State the clinical significance of abnormal results obtained, correlating patient results as to possible disease and /or therapy states.
- Apply normal ranges utilized by the department to correctly interpret patient results as normal or abnormal.
- Brings abnormal results to the attention of the clinical instructor/preceptor
- Recognizes critical values and states action which must be taken when such a value is obtained on a patient sample.
- Identify and differentiate the common cell types found in peripheral blood.
- Complete 10 normal differentials within ± 20% accuracy of the counts performed by automated method.
- Complete 10 reviews of RBC morphology within ± 20% accuracy of the clinical instructor/preceptor.
- Recognize immature and abnormal cells in peripheral blood.
- Complete 10 abnormal manual differentials within ± 20% accuracy of the clinical instructor/preceptor.
- Correlate each manual differential to the automated results including platelet, WBC, differential and indices.
- Calculate the corrected WBC when nucleated red blood cells are present.
- State the action to be taken when automated and manual results do not agree.
- State the significance of certain blood cells and RBC morphology associated with clinical disease.
- Reviews criteria for pathology review of blood smears.
- Set up and read 5 acceptable Erythrocyte Sedimentation Rates
- Reticulocyte Counts – 5 manual (Discuss only if not performed manually; if manual procedure performed, student should achieve level 5)
- Prepares sample with staining solution.
- Prepares a readable smear
- Performs the reticulocyte count.
- Reports results using appropriate units.
- Correctly evaluates gross appearance and color including xanthochromia, if relevant.
- Performs cell counts.
- Accurately performs dilutions as necessary.
- Accurately calculates results on diluted samples.
- Completes differentials with 90% accuracy.
- Discuss the type and significance of crystals found in synovial fluid.
- Discuss the process for referral to pathologist for review.
- Bone Marrow (Observe Preparation and Slide Review)
- Malarial Smear
- Sickle Cell Prep
Urinalysis
26
Urinalysis
26
- Locate and read the Urinalysis procedure manual
- Process incoming specimens for testing
- Properly dispose of finished specimen
- Demonstrate efficient and careful use of light microscope
- Set up the urinalysis analyzer
- Run controls on the urinalysis analyzer and determine proper course of action if out of range
- Run patients on the urinalysis analyzer
- Perform manual urine chemstrip analysis
- Perform a microscopic examination of urine sediment, properly identifying all cells, casts, crystals, bacteria, and miscellaneous material
- Perform a urine pregnancy test
- Perform a serum pregnancy test
- Perform departmental QC and record
- Maintain proper departmental paperwork
- Document appropriate course of action if controls are out of range
- Observe a gastroccult test
- Discuss a galactosemia screen
- Discuss testing of glucose tolerance urines
- Discuss Clinitest
- Discuss Ictotest
- Discuss a test for Bence-Jones protein in urine
- Discuss principles of operation and routine maintenance of the urinalysis analyzer
- Discuss specific gravity test on the refractometer
- Discuss checking the accuracy of the refractometer
- Discuss calibration of the refractometer
- Discuss semen analysis and observe if possible
- Discuss laboratory studies for suspected rape cases
Microbiology
40
Microbiology
40
- Locate and read the Microbiology procedure manual as the rotation progresses.
- Handle incoming culture specimens.
- Log in incoming specimens.
- Inoculate specimens onto appropriate media.
- Prepare and stain slides with Gram stain.
- Select proper media for subculturing a specific unknown bacterial colony.
- Perform coagulase testing.
- Perform oxidase testing.
- Perform departmental QC procedures for media, reagents, temperatures, etc.
- Perform optochin testing for identification of pneumococci
- Perform appropriate biochemical testing for a specific bacterial colony. (indole, citrate, hippurate, Dnase, urea)
- Perform Kirby-Bauer antibiotic susceptibility testing.
- Perform B-lactamase testing.
- Perform bacterial identification using manual or automated systems.
- Use incubation system to recover anaerobic bacteria.
- Perform processing of Hemoccult slides.
- Observe Clostridium difficile testing.
- Observe scotch-tape preparations for pinworm testing.
- Observe blood culture inoculation.
- Observe subculturing procedures for positive blood cultures.
- Observe KOH preparations.
- Observe testing procedures for Chlamydia, Cryptococcal antigens, Rotavirus, and antinuclear antibodies.
- Discuss principles of operation and routine maintenance automated system.
- Discuss state health reporting procedures.
- Wet prep from cervical/urethral discharge
- Stool for Fecal Fat
- Stool for Leukocytes
- Formalin/Ethyl Acetate Concentration (Ova and Parasites)
- Zinc Sulfate floation
- Making PVA permanent smear (Ova and Parasites)
- Trichrome Stain (Ova and Parasites)
- Giemsa for Blood parasites
- Preparation of Direct Smear & smear from concentrated stool
- Acid-Fast smear – Carbol fuchsin
- Acid-Fast culture
- Yeast Identification – Candida albicans
- Yeast fermentation kits
- Fungus culture
- Slide culture for fungal identification
- Tease mount for fungal identification
Serology
7
Serology
7
- Locate and read the serology procedure manual.
- Identify patient specimens appropriately.
- Selects proper supplies and uses appropriately.
- Perform RPR card test.
- Test:
- Perform departmental quality control.
- Maintain appropriate departmental paperwork.
Related Instruction Content
Training Provider(s):
Davidson-Davie Community College
Art Appreciation
Art Appreciation
Writing and Inquiry
Writing and Inquiry
Professional Research and Reporting
Professional Research and Reporting
General Psychology
General Psychology
Basic Anatomy and Physiology
Basic Anatomy and Physiology
Introduction to MLT
Introduction to MLT
Statistical Methods I
Statistical Methods I
Introduction to Microbiology
Introduction to Microbiology
General, Organic, and Biochemistry
General, Organic, and Biochemistry
General, Organic, and Biochemistry Lab
General, Organic, and Biochemistry Lab
Urinalysis and Body Fluids
Urinalysis and Body Fluids
Hematology/Hemostasis I
Hematology/Hemostasis I
Immunohematology
Immunohematology
Hematology/Hemostasis II
Hematology/Hemostasis II
Clinical Chemistry I
Clinical Chemistry I
Professional Issues
Professional Issues
MLT Practicum I
MLT Practicum I
Special Clinical Microbiology
Special Clinical Microbiology
Professional Issues
Professional Issues
MLT Practicum II
MLT Practicum II